Product Name: | Mouse coagulation factor VIIIa light chain (F8aLC) ELISA kit |
Product Type : | ELISA kit |
Code : | CSB-EQ027659MO |
Size : | 96T,5×96T,10×96T |
Abbreviation : | F8aLC |
Species : | Mouse |
Sample Types : | serum, plasma, tissue homogenates |
Detect Range : | 1.56 ng/ml-100 ng/ml |
Sensitivity : | 0.39 ng/ml |
Assay Time : | 1-3h |
Sample Volume : | 50-100ul |
Detection Wavelengt : | 450nm |
Research Area : | Others |
Protocol : | |
Precision : | Intra-assay Precision (Precision within an assay): CV%<8%Three samples of known concentration were tested twenty times on one plate to assess.Inter-assay Precision (Precision between assays): CV%<10%Three samples of known concentration were tested in twenty assays to assess. |
Linearity : | To assess the linearity of the assay, samples were spiked with high concentrations of mouse F8aLC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. SampleSerum(n=4)1:100Average %106Range %103-1091:200Average %87Range %83-901:400Average %107Range %103-1101:800Average %96Range %93-99 |
Recovery : | The recovery of mouse F8aLC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.Sample TypeAverage % RecoveryRangeSerum (n=5)108104-112EDTA plasma (n=4)9289-95 |
Typical Data : | These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.ng/mlOD1OD2Average1000.0990.0980.099500.2040.2100.207250.3490.3290.33912.50.5040.5120.5086.250.7660.7720.7693.121.0061.0631.0351.561.3501.4001.37502.1672.2002.184 |
Mouse Coagulation Factor VIIIa Light Chain (F8aLC) ELISA Kit